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Microsopy, Atomic force, Confocal, Epifluoresent

Microscope - Atomic Force: Digital Instruments Bioscope Atomic Force Microscope
Department : Department of Biomedical Engineering, Facility of Confocal Imaging

EMSL's Digital Instruments BioScope™ Atomic Force Microscope (AFM) combines optical and AFM techniques used to examine heterophase processes on mineral surfaces and to image biological systems, such as bacterial colonies, on minerals and other substrates.

Microscope - Confocal: Leica TCS SP2 Workstation
Department : Department of Biomedical Engineering, Facility of Confocal Imaging
  • Argon-Krypton Laser for Light Emission at 488nm (blue), 568nm (green), and 647nm (red)
  • Fluorescence Emission Filters: Long Pass (LP) 515, 520, and 590nm; Band Pass (BP) 575-640, 590-610, and 670-810nm
  • Photomultiplier Tube (PMT) detectors for imaging three channels (any combination of two fluorescent modes, and a reflected or transmitted mode)
  • Objectives for 10x, 20x, 40x, 63x (dry and glycerin immersion) and 100x (oil immersion)
  • Differential Interference Contrast (DIC) optics

Microscope - Confocal: Zeiss LSM 710 Confocal Microscope
Department : School of Environmental and Biological Science

Laser lines
The LSM 710 is capable of 34 spectral detection channels using six laser lines - 405, 458, 488, 514, 561 & 633 nm, plus an additional 594 nm HeNe laser (includes replacement main beam splitter wheel equipped for 458, 488, 514, 561, 594 & 633 nm laser lines). The 561 and 594 lasers are needed to visualize mCherry, Texas Red, and other red fluorophores including Alexa 568, Alexa 594.

Microscope - Epifluorescent: Nikon Eclipse TE2000-S
Department : Department of Biomedical Engineering, Facility of Confocal Imaging

 

  • X-Cite® 120Q system uses a 120-watt lamp to deliver rich spectral excitation with a uniformly illuminated field of view. The optional adjustable iris allows the user to set the level of illumination.

  • 3 fluorescence filter blocks: Ultraviolet Excitation (UV-2E/C) Ex 340-380 and Em 435-485, Blue Excitation (UV-2E/C) Ex 465-495 and Em 515-555, Yellow Excitation (Y-2E/C-TR) Ex 540-580 and Em 600-660

  • Objectives for 4x, 10x, 20x, 40x, and 100x magnification

  • Differential Interference Contrast (DIC) optics

Microscope - STEM: JEOL 2010F
Department : Institute for Advanced Materials, Devices and Nanotechnology (IAMDN)

Field Emission TEM/STEM, (JEOL 2010F)

  • Acceleration voltage 20-200kV with ZrO-W field emission source
  • Scanning Transmission Electron Microscopy (STEM) interface
  • Digital imaging with 1Kx1K CCD camera
  • BF/DF STEM detectors
  • HADF STEM detector
  • Electron Energy Loss (EELS) spectrometer
  • 2Kx2K GATAN imaging EELS filter (GIF)
  • Heating (1400K) and cooling (90K) holders
  • Electron biprism for holography imaging of magnetic and electrical fields