BioTek Synergy™ 4 is a modular microplate reader that offers Monochromator-Based Fluorescence Intensity (M); Filter-Based Top and Bottom Fluorescence Intensity (F); Fluorescence Polarization (P); Time-Resolved Fluorescence (T); Luminescence (L); UV-Visible Absorbance (A); and Dual Reagent Injection System (D). With its unique Hybrid Technology TM optics, the Synergy 4 covers more applications than any other reader on the market. The hybrid fluorescence system comes with two double-grating monochromators, two excitation filters (360/40, 485/20 nm default), three emission filters (460/40, 528/20, 620/40 nm default) and three mirrors (50%, 400 nm half-size and 510 nm full-size default). Temperature control system and shaking are standard.

Additional filter sets include: 485/20 EX (EGFP), 516/20 EM (EGFP), 540/35 EX (RFP), 600/40 EM (RFP), 360/40 EX (DAPI), 460/40 EM (DAPI), 590/20 EX (Texas Red), 645/40 EM (Texas Red)